Fig. 5.
CD40 and IgM crosslinking result in transcription of mcl-1, bcl-2, and bfl-1 in CLL B cells.
(A) CLL cells were purified from the peripheral blood of an untreated patient and RNAs isolated from unstimulated cells (t0) or after 4 hours with media only, anti-CD40 mAb, anti-IgM, or dexamethasone. Expression of mRNA encoding specific bcl-2 family members was measured by RPA. The data shown represent those of 5 similar analyses. (B) Densitometric evaluation of these RPA results, with fold induction based on comparison with the L32 signal for each lane.
