Steady-state kinetics of wild-type and mutant R510Q RPK.

(A) Steady-state kinetics of recombinant wild-type (circles) and R510Q mutant (squares) RPK as a function of PEP. All experiments were performed at 37°C according to the published method.20Open symbols represent data points obtained in the presence of 1 mM FBP and filled symbols obtained without FBP. (B) Kinetics of wild-type (circles) and R510Q (squares) PKs against ADP. Concentration of PEP was fixed at 5 mM.