SDS-PAGE and analytical gel chromatography of recombinant human RPK.

(A) SDS-PAGE of purified recombinant human RPK. Three samples with increasing protein concentrations were run in parallel with molecular mass standards on a 10% gel and stained with Coomassie Blue R-250. (B) Elution profile of RPK from a Superose 12 HR 10/30 column. The position of the peak corresponds to a protein of 240 kDa, indicating a tetramer. Catalase (240 kDa), aldolase (158 kDa), albumin (68 kDa), ovalbumin (45 kDa), chymotrypsinogen (25 kDa), and cytochrome c (12 kDa) were used as molecular mass standards.