Immunophenotypic pattern of IFN-DCs as compared with IL-4-DCs.

Highly purified CD14⁺ monocytes were cultured with GM-CSF and either IL-4 or type I IFN for 3 days, stained with a panel of antibodies, and analyzed by flow cytometry. (A) This panel shows the representative dot histogram FACS profiles obtained with monocytes isolated from 1 of 4 donors, whose DC cultures were tested on day 3. Relative profiles obtained with isotype-matched control antibodies (dotted lines) are shown. (B) This panel reports the mean percentages of positive cells and of mean fluorescence intensity (MFI) obtained in 4 experiments performed with monocytes from different donors.