Fig. 5.
![Fig. 5. JRFL gp120 induces MAPK activation in macrophages. / MDMs were stimulated with JRFL gp120 (2.5 μg/mL) or MIP-1β (200 nM) for the indicated time periods. Cell lysates were resolved by 12% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblot analysis with antibodies specific for the total or phosphorylated forms of (A) p38 MAPK (phospho-p38 MAPK [T183/Y185] antibody); (B) JNK/SAPK (phospho-JNK/SAPK [T180/Y182] antibody); and (C) ERK1/2 (p44/42) (phospho-p44/42 MAPK [T202/Y204] antibody). Blots shown are from one donor and are representative of 5 experiments using different donors that yielded similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/10/10.1182_blood.v98.10.2909/5/h82211757005.jpeg?Expires=1724264908&Signature=BclM98Rwiel3XLIYICPEtzPGm1lCn-4LCLi8QPqk48xF49MRLrL~Y~jyT1nH~9IdMCQyqbMB-l8mu9mSg8kjbpzFWop5IE1V7sltXAevNYNZ1vHefZXI~3u9dZOdItEfm6f4gmZv9GHD0rmStxjmHsucP3nqbL9oMc-m-giMF5pHAl6t5bAqhBGg0BL22MNmCHf76DKrXE9hJZS3GFaoDGldH7xSntJZrI7XxDQma8Xrm-QtGs1nZuO0H~ftUxezp0aIl3F8QdcyenI4qZIbmxN5F7-RTlAmm4997dZjWhoJuwjAmucPGqLNGv9GHUPHmUd7g5QQ9WO5jSysDa6a6A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
JRFL gp120 induces MAPK activation in macrophages.
MDMs were stimulated with JRFL gp120 (2.5 μg/mL) or MIP-1β (200 nM) for the indicated time periods. Cell lysates were resolved by 12% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblot analysis with antibodies specific for the total or phosphorylated forms of (A) p38 MAPK (phospho-p38 MAPK [T183/Y185] antibody); (B) JNK/SAPK (phospho-JNK/SAPK [T180/Y182] antibody); and (C) ERK1/2 (p44/42) (phospho-p44/42 MAPK [T202/Y204] antibody). Blots shown are from one donor and are representative of 5 experiments using different donors that yielded similar results.
