![Fig. 1. Tyrosine phosphorylation of Pyk2 in primary human macrophages occurs in response to HIV-1 R5 Env. / (A) Week-old cultures of MDMs were unstimulated (CTR) or exposed for 5 minutes to JRFL gp120 (2.5 μg/mL), lysed, and subjected to immunoprecipitation with an anti-Pyk2 antibody. Immune complexes were resolved on 8% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to serial immunoblotting with an antiphosphotyrosine 4G10 antibody (upper panel) and an anti-Pyk2 antibody (lower panel). (B) MDMs were unstimulated (CTR) or exposed to JRFL gp120 (2.5 μg/mL) for the indicated times (minutes). Cell lysates were resolved by 8% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with anti-Pyk2 phosphospecific [pY402] antibody (upper panel, P-Pyk2). The same blot was then reprobed with an antibody specific for total Pyk2 (lower panel). Blots shown are representative of 5 independent experiments using different donors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/10/10.1182_blood.v98.10.2909/5/h82211757001.jpeg?Expires=1724265656&Signature=YefuOVH-mkp9jFVkWkA4uLidIaCq2Dg6IC-aCy74OSAK-szcAXTtMdPx49C8i6hbvZWFFNqfNzh~GMLX~yjMSGqqlsWW2jGxYhA2Ns5qJWXWfSfRQKuASsmWuBxD39wu6ov6d3i6rDbEKXkEQ-Tiy9T9MbELtR7ehXLokiEM~05v9RhDKqZ197QNI39TqHpBo3uecklg6paWUu14fordbd0hSQMT757SxIzzT4Ai7BlG1TLQvuwqqTnBHyvAlskDB0K~ja8DL3faRBg3CNtHmamNDMr-~CT~sNXa7kBSHqhd-IDPc-JrtAJ66ELja5KWamEebwYCxg9KHTMx5yIqvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Tyrosine phosphorylation of Pyk2 in primary human macrophages occurs in response to HIV-1 R5 Env.
(A) Week-old cultures of MDMs were unstimulated (CTR) or exposed for 5 minutes to JRFL gp120 (2.5 μg/mL), lysed, and subjected to immunoprecipitation with an anti-Pyk2 antibody. Immune complexes were resolved on 8% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to serial immunoblotting with an antiphosphotyrosine 4G10 antibody (upper panel) and an anti-Pyk2 antibody (lower panel). (B) MDMs were unstimulated (CTR) or exposed to JRFL gp120 (2.5 μg/mL) for the indicated times (minutes). Cell lysates were resolved by 8% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with anti-Pyk2 phosphospecific [pY402] antibody (upper panel, P-Pyk2). The same blot was then reprobed with an antibody specific for total Pyk2 (lower panel). Blots shown are representative of 5 independent experiments using different donors.
