Donor spleen cell infusions induce potent graft-versus-leukemia effects in mixed chimeras conditioned with Flu-XRT-Cy.
Nonmyeloablative allogeneic stem cell transplantation (NST) was performed by infusing 20 million B10.BR marrow cells on day 0 into B10 mice conditioned with fludarabine 100 mg/kg/day on days −6 to −2, with 200 cGy TBI on day −1, and with Cy 200 mg/kg intraperitoneally on day 3. On day 21, animals who had received transplants received nothing (▵; n = 5) or donor lymphocyte infusion (DLI) consisting of 20 million B10.BR spleen cells IV (●; n = 10). On day 30, all recipients of NST and a group of 5 B10 mice that hadn't received transplants (▪) received an intravenous injection of 2 × 105 C1498 leukemia cells, of B6 origin. Survival was monitored thrice weekly.
