Expression of WASP and N-WASP in human blood cells examined by Western blot.

Cell lysates were stained with N-WASP Ab-1. (A) HeLa cells and normal human PBMCs. Blots show WASP and N-WASP (low levels) in PBMCs. WASP is absent as anticipated in HeLa cells. Bands at 74 to 80 kd (∗) may represent proteins of the SCAR/WAVE family.17 18 (B) Verification of N-WASP presence in normal platelets by immune precipitation. WASP and N-WASP were precipitated from normal platelet lysate by N-WASP Ab-2 and detected with N-WASP Ab-1. Normal rabbit IgG failed to precipitate detectable bands. (C) Blood cells and platelets from patients and control subjects. N-WASP was detected at similar levels in PBMCs, neutrophils (Neut), EBV-transformed B-cell lines (BCL) (upper panel), and platelets (lower panel) of various healthy control subjects (all indicated by N) and WAS patients (P₁ to P₆).