Fig. 3.
Thermal stability of TPI at 52°C detected by enzyme activity. Effect of MTs and IOVs on the stability of mTPI.
Decays were fitted by single exponentials as shown by solid lines. (A) Recombinant mTPI (○) and wTPI (●), characterized by t0.5 = 7.0 ± 0.4 minutes and t0.5 = 51.1 ± 7.3 minutes, respectively. (B) TPI activities of hemolyzates from the propositus (■) and control (▪), characterized by t0.5 = 6.3 ± 1.4 minutes and t0.5 = 63.5 ± 5.8 minutes, respectively. (C) Activity of mTPI in the presence of MTs (▵) or IOVs (▴); activity is the reduced activity of mTPI due to the binding to MTs or IOVs.
