Rare event analysis of human CD123⁺ and CD11c⁺ DCs by flow cytometry.

PBMCs were selected in the R1 gate excluding debris and polynuclear cells (A). Lineage-negative cells were selected in the R2 gate (B). In this combined R1 and R2 gate, CD11c⁺, HLA-DR⁺⁺(C, R4) or CD123⁺, HLA-DR⁺ (D, R3) events were quantified.