Rabaptin-5/PDGFβR retrovirally transduced into murine bone marrow causes a fatal myeloproliferative disease.

(A) Survival of lethally irradiated mice receiving bone marrow transplants transduced with empty plasmid, Rabaptin-5/PDGFβR (R/P), or tel-PDGFβR (T/P) containing retroviruses. (B) Southern blot of splenic genomic DNA using an eGFP probe demonstrating proviral integration with several bands detected in samples from 6 mice receiving transplants with Rabaptin-5/PDGFβR (R/P) and 3 mice with tel/PDGFβR. (C) A representative peripheral blood smear from a mouse with the rabaptin-5/PDGFβR-mediated myeloproliferative disease (× 400) revealing marked leukocytosis with predominantly mature granulocytes. (D) Formalin-fixed, hematoxylin and eosin-stained spleen sections from a mouse with Rabaptin-5/PDGFβR-mediated myeloproliferative disease (× 400) revealing near total replacement of normal spleen architecture by mature granulocytes.