Rabaptin-5/PDGFβR transforms Ba/F3 cells to growth factor independence.

(A) Ba/F3 cells were infected with bicistronic MSCV retroviral vectors containing eGFP alone (empty virus, circle) or eGFP along with Rabaptin-5/PDGFβR (box), tel/PDGFβR (diamond), or the kinase-inactive mutant of Rabaptin-5/PDGFβR (triangle). After infection, cells were flow sorted for eGFP positivity, expanded, and subsequently incubated in media with (open symbols) or without (closed symbols) IL-3, 1 ng/mL. The data represent the mean of 3 independent cultures. (B) Presence of expressed fusion protein in Ba/F3 cell lines transformed with either Rabaptin-5/PDGFβR (R/P) or tel/PDGFβR (T/P). Membranes were immunoblotted using an anti-PDGFβR (C-terminal) antibody.