RT-PCR identifies the patient specific 5′ Rabaptin-5/PDGFβR 3′ fusion breakpoint, but not the reciprocal breakpoint.

RT-PCR was carried out on patient myeloid cell total RNA and normal donor bone marrow stroma total RNA, using primers to identify the 2 potential breakpoint products, 5′ Rabaptin-5/PDGFβR 3′ and 5′ PDGFβR/Rabaptin-5 3′, as well as the 2 involved native gene products.