Fig. 6.
Adhesion of U937 cells to αEC and D100.
(A) U937 cells in HBSS were labeled with Calcein and activated with 10 nM PMA. Aliquots of 5 × 105cells/mL were then added to the wells coated with αEC (●) and D100(▿). (B) Effect of mAbs on adhesion to αEC. Activated U937 cells were preincubated with either mAb 44a, mAb 3.9, or mAb IB4 alone (20 μg/mL), with a combination of 44a and 3.9 or with 4 mM EDTA for 15 minutes at 22°C. Then, aliquots of 5 × 104cells were added to microtiter plates coated with 10 μg/mL αEC.
