Fig. 2.
Fig. 2. Adhesion of cells transfected with αMβ2 to recombinant αEC and γC. / (A) αMβ2-transfected HEK 293 cells (5 × 104) labeled with Calcein AM in HBSS and HEPES were added to the wells of 48-well, tissue culture–treated microtiter plates coated with different concentrations of the recombinant αEC (●) and γC (▾) domains and postcoated with 1% PVP. After 25 minutes at 37°C, nonadherent cells were removed by 3 washes with phosphate-buffered saline. Fluorescence of adherent cells was measured in a fluorescence plate reader and converted to cell number. Adhesion of mock-transfected cells (▪) to αEC is shown for comparison. Results are the mean ± SE values from 3 individual experiments done in triplicate. (B) Microscopical study (original magnification, × 20) of the cells adherent to the control (PVP), γC, and αEC.

Adhesion of cells transfected with αMβ2 to recombinant αEC and γC.

(A) αMβ2-transfected HEK 293 cells (5 × 104) labeled with Calcein AM in HBSS and HEPES were added to the wells of 48-well, tissue culture–treated microtiter plates coated with different concentrations of the recombinant αEC (●) and γC (▾) domains and postcoated with 1% PVP. After 25 minutes at 37°C, nonadherent cells were removed by 3 washes with phosphate-buffered saline. Fluorescence of adherent cells was measured in a fluorescence plate reader and converted to cell number. Adhesion of mock-transfected cells (▪) to αEC is shown for comparison. Results are the mean ± SE values from 3 individual experiments done in triplicate. (B) Microscopical study (original magnification, × 20) of the cells adherent to the control (PVP), γC, and αEC.

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