Capacity for generation of p65 NFκB within H7 cells containing either p210BCR-ABL or gag-akt/RafCAAX is severely inhibited by treatment of cells with parthenolide for 4 hours, whereas Sp1 transcription factor binding activity is unaffected.

Lysates were made of the noted cells treated for 4 hours with parthenolide, 40 μM, and then gel shifts of equal quantities of protein were performed with a radiolabeled NFκB oligonucleotide probe.