Parthenolide, a specific NFκB inhibitor, produces apoptosis in H7gag-akt/RafCAAX cells, but is inactive in this regard in the sister cells transformed by p210BCR-ABL.

The aforementioned cells were treated or not with parthenolide, 10 μM, for 24 hours, and then apoptosis was measured by flow cytometric analysis of annexin V binding and propidium iodide uptake (A) or by analysis of PARP cleavage on immunoblot (B). In (A), apoptotic cells are those in the upper right quadrant with dual staining with propidium iodide and annexin V. In (B), the 85-kd degradation product of caspase-cleaved whole PARP (118 kd) is observed after parthenolide treatment of H7gag-akt/RafCAAX.