Withdrawal of estrogen from EPΔ578ER progenitors initiates a transcriptional program of myeloid differentiation.

Northern blot analysis was performed on RNA samples prepared from EPΔ578ER progenitors maintained in estrogen (+β-E) as well as cultured out of estrogen (−β-E) for 1 to 9 days. RNA from NIH3T3 fibroblasts was used as a nonmyeloid control. In general, transcription factor probes were hybridized to blots prepared from polyA⁺-RNA, whereas other probes were hybridized to blots prepared from total RNA. Names of the probes are indicated to the left of each blot. Equivalent loading was verified by hybridization of a β-actin probe.