American Society of Hematology

Fig. 4.

$Fig. 4. IFN-α and -β induce cyt c release and Δψm reduction. / (A) Cyt c levels were determined in cellular fractions isolated by differential centrifugation from control or IFN-treated U266 cells as described in “Materials and methods.” Immunoblotting with anti–cyt c mAb and β-actin as a control were used to determine the levels of cyt c in the S100 fractions, representing the cytosolic cyt c in 20 μg of cellular protein. (B) The Δψm was determined by using 10 nM DIOC6 (3) as described in “Materials and methods.” The percentage of Δψm (low) after the cell debris were excluded was determined, and comparative experiments were performed on the same day. The data shown are representative of 3 separate duplicate experiments.$

IFN-α and -β induce cyt c release and Δψ_m reduction.

(A) Cyt c levels were determined in cellular fractions isolated by differential centrifugation from control or IFN-treated U266 cells as described in “Materials and methods.” Immunoblotting with anti–cyt c mAb and β-actin as a control were used to determine the levels of cyt c in the S100 fractions, representing the cytosolic cyt c in 20 μg of cellular protein. (B) The Δψ_m was determined by using 10 nM DIOC₆ (3) as described in “Materials and methods.” The percentage of Δψ_m (low) after the cell debris were excluded was determined, and comparative experiments were performed on the same day. The data shown are representative of 3 separate duplicate experiments.

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