Flow cytometric analysis of 4G4 transductants.

4G4 T cells were transduced with retroviral supernatants. Data for construct 5 (K^b-ζ-CD4) and construct 7 (K^b-ζ-CD28) show unsorted primary transductants. Because of the poor surface expression of these constructs, they were not further analyzed. Data for other constructs show GFP-sorted populations. Mean fluorescent intensities for chimeric receptor (K^b) expression among GFP-positive cells (total cells for 4G4-untransfected) are listed within each plot. Relative expression levels were consistently observed with multiple transductions of each construct.