Fig. 1.
Chimeric construct structure.
Constructs were created by linking components in a cassette fashion (A). Amino acid sequence at the intersection of each component is noted (B). Additional amino acids added at the junction between components result from the insertion of restriction enzyme sites required for construct synthesis. Extracellular and transmembrane domains of all constructs are derived from the MHC class I H-2 Kbmolecule. The cytoplasmic domains are as listed. Construct 2 truncates the ζ cytoplasmic domain after the first ITAM.
