Fig. 5.
![Fig. 5. IFN-γ production by CD8+CD44hi cells in vaccinated mice. / Using flow cytometry-bound assessment of cytokine production on day 21, phenotypically high expression of IFN-γ was demonstrated in either IL-10–administered or antigen-challenged groups (groups 2-4) (left). However, the absolute number of IFN-γ–producing CD8+CD44hi cells in spleen persisted in groups 2 and 3 through day 28. (On day 21, group 3 vs group 1 [P < .05]; group 3 vs group 2 [P > .05]; group 3 vs group 4 [P < .05]; on day 28, groups 1, 4 vs group 3 [P < .0001]; group 2 vs group 3 [P < .01]). The absolute number of IFN-γ–secreting T cells in spleen was calculated as (percentage × spleen cell number). Mean values are shown for 3 mice at each time point.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/7/10.1182_blood.v98.7.2143/5/h81911575005.jpeg?Expires=1722386432&Signature=cj8JdYHks5oIqC-ICqDfYNozGxO0icwlGxGCVjWjWTRe6DEsfojKiuQaI5bu07PbcHJy3q25gsTmYZgp5C~Bpa6oyIbZ03rAoGqfxp8AnQN2FgCIPvHXmVRATX97RYBpxXaGtLysDK91DBX9sMskjgNDJvQfLIcNogcgmZq08B52NuefLaEx78IWJU~4SOZ37heBExNU31naH0T1frEKubRCllUF8DBOaAWg9GrYzYP9~uQHIZ0LjrVMbBaq-HCiXL1tkB9Joyv5GFILybfJVK0rHOd-1aULOQerFXKEGCoGOtNhmZfmruulKANnzcmkCgjPwxRoYJbYU7f~BGeB8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IFN-γ production by CD8+CD44hi cells in vaccinated mice.
Using flow cytometry-bound assessment of cytokine production on day 21, phenotypically high expression of IFN-γ was demonstrated in either IL-10–administered or antigen-challenged groups (groups 2-4) (left). However, the absolute number of IFN-γ–producing CD8+CD44hi cells in spleen persisted in groups 2 and 3 through day 28. (On day 21, group 3 vs group 1 [P < .05]; group 3 vs group 2 [P > .05]; group 3 vs group 4 [P < .05]; on day 28, groups 1, 4 vs group 3 [P < .0001]; group 2 vs group 3 [P < .01]). The absolute number of IFN-γ–secreting T cells in spleen was calculated as (percentage × spleen cell number). Mean values are shown for 3 mice at each time point.
