Fig. 4.
![Fig. 4. Assessment of memory type CD8+ T cells in vaccinated mice. / After vaccination, the number of CD8+ T cells expressing activated–memory phenotype (CD44hi CD122+) were estimated by flow cytometry. On day 21, cells expressing these phenotypes increased in IL-10–treated mice (groups 3 and 4) (left), and the absolute number in spleen increased until day 28 (right). (On day 21, group 3 vs group 1, [P < .05]; group 3 vs group 2 [P < .01]; group 3 vs group 4 [P > .05]). The absolute number of such T cells in spleen was thus calculated as (percentage × spleen cell number). Mean values are shown for 3 mice evaluated at each time point.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/7/10.1182_blood.v98.7.2143/5/h81911575004.jpeg?Expires=1722387066&Signature=k1gHl7VzVinMFdjGkWbzF2KeDjR6yWvQ3ViNjfehf-RKhHencsgtHa4a3qNbkK8-3lBVaHzF-~qXFeAxYxseuGgu3lWY0WqCvzh-7T-cY~HpiQvgPVKhsqjJf-HbMFYV3uGQWZVErs174UlXUSc3HvOWmR67hsoW1cVmgYBxB1MVWFOpLfB6dq6ZjSth9PaDK199U3yUJWmkyptOUUHRtbi3OUhulUan9XIEtInYJxtq~HePlSpQMhAqRGqxgu4ayoBHD8~YO5ebhMtid3rhP44EUz30J8M8KEKkoZLfSZHREyk0feYvo2JURYyem5fXcsuWPpGIO1EdyDyMeZ-xzA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Assessment of memory type CD8+ T cells in vaccinated mice.
After vaccination, the number of CD8+ T cells expressing activated–memory phenotype (CD44hi CD122+) were estimated by flow cytometry. On day 21, cells expressing these phenotypes increased in IL-10–treated mice (groups 3 and 4) (left), and the absolute number in spleen increased until day 28 (right). (On day 21, group 3 vs group 1, [P < .05]; group 3 vs group 2 [P < .01]; group 3 vs group 4 [P > .05]). The absolute number of such T cells in spleen was thus calculated as (percentage × spleen cell number). Mean values are shown for 3 mice evaluated at each time point.
