Fig. 3.
![Fig. 3. Cell proliferation over time with and without IL-10 in the vaccination models. / Proliferation assays were performed in the presence and absence of antigen, cytokines (100 U/mL of IL-2 and IL-15, respectively) or PMA–ionomycin (PMA, 25 ng/mL; ionomycin, 0.5 μM) using splenocytes (1 × 106 cells/well) harvested from mice 14, 21, and 28 days after DC vaccination in each group depicted in Figure 2. After 48-hour culture, 0.037 MBq (1 μCi) [3H]-thymidine was added, and cells were harvested 16 hours later. Statistically significant differences relative to DC-vaccinated mice that did not receive IL-10 are indicated. Control mice were immunized with DCs in the absence of peptide. Each bar represents the mean of 3 mice evaluated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/7/10.1182_blood.v98.7.2143/5/h81911575003.jpeg?Expires=1722385797&Signature=gFcMtbwGw0GX-7fY8hW7rbf7fLSi-kAhofVXYRP-QD39rUxLgQGh21Qr914nsgTeYbxwVtHe9jmEkEva0hm8tThaG7b~Kmq8jjugbZuXk9MBYeOU4Q7zMGGYY-xu0ujpj4VjyW6QcpBj6E0FVD8R0DrtEwRUlOS1~LTDQizNt7v73kP6QMUfTGVDzvO9anZ118qllQI96q~9IkPRW3EwlHPEBbgTdVA65~VDJhw9av51ViJzuZwFU4wLf2ECEAAoMckkPhUn3xVRztJraZ0~z-6WbsVpFnP9JRuqgCcyQzv8wnS~AIDnkYrZxZ4uigk5Z~4ywH4TmWs1WXVbxeerSg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell proliferation over time with and without IL-10 in the vaccination models.
Proliferation assays were performed in the presence and absence of antigen, cytokines (100 U/mL of IL-2 and IL-15, respectively) or PMA–ionomycin (PMA, 25 ng/mL; ionomycin, 0.5 μM) using splenocytes (1 × 106 cells/well) harvested from mice 14, 21, and 28 days after DC vaccination in each group depicted in Figure 2. After 48-hour culture, 0.037 MBq (1 μCi) [3H]-thymidine was added, and cells were harvested 16 hours later. Statistically significant differences relative to DC-vaccinated mice that did not receive IL-10 are indicated. Control mice were immunized with DCs in the absence of peptide. Each bar represents the mean of 3 mice evaluated.
