ERK 1/2 and p38 MAPK differentially affect the levels of IκBα during maturation of MDDC.

Immature MDDC were not treated (control) or were cultured with TNF-α for 24 or 48 hours and in either the absence (minus sign) or presence of inhibitor (PD indicates PD98059; and SB, SB203580). Cytoplasmic extract (10 μg) from each culture condition was subjected to Western blotting using a rabbit polyclonal antiserum specific for IκBα. The experiment was done with samples from 2 different donors and identical results were obtained; a representative experiment is shown.