IL-12 p40 and p70 production by TNF-α–treated and LPS-treated MDDC in the presence of ERK or p38 MAPK signaling-pathway inhibitors.

Immature MDDC were not treated (control) or were stimulated with either TNF-α (20 ng/mL; A) or LPS (10 ng/mL; B-D) for 24 hours (B), 48 hours (A,C), or the indicated times (D), and IL-12 p40 and IL-12 p70 release was determined by enzyme-linked immunosorbent assay. When PD98059, U0126, or SB203580 were used, cells were preincubated for 1 hour before stimulation. Each assessment was done at least 3 times. In panels A to C, mean and SD results from a representative experiment are shown.