Fig. 9.
Fig. 9. Effect of TNFR1 and TNFR2 on the stability of TNF-α mRNA. / BMSCs were prepared as described in “Materials and methods” and were stimulated with 1 μg/mL LPS for 2 hours. After that, actinomycin D (5 μg/mL) was added and cells were harvested for RNA at the indicated times. Northern blots from these experiments are shown at the top, with the TNF-α and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs indicated. On the bottom is the PhosphorImager quantitation of the TNF-α mRNA levels after normalization to GAPDH mRNA levels. The absence of either TNFR1 (TNFR1KO) or TNFR2 (TNFR2KO) did not affect the half-life of TNF-α mRNA. However, when TTP was also absent (TTP/TNFR1KO, TTP/TNFR2KO), the stability of TNF-α mRNA was increased, particularly when both TTP and TNFR2 were absent. The experiment shown here is representative of 2 independent experiments with cells derived from different groups of animals.

Effect of TNFR1 and TNFR2 on the stability of TNF-α mRNA.

BMSCs were prepared as described in “Materials and methods” and were stimulated with 1 μg/mL LPS for 2 hours. After that, actinomycin D (5 μg/mL) was added and cells were harvested for RNA at the indicated times. Northern blots from these experiments are shown at the top, with the TNF-α and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs indicated. On the bottom is the PhosphorImager quantitation of the TNF-α mRNA levels after normalization to GAPDH mRNA levels. The absence of either TNFR1 (TNFR1KO) or TNFR2 (TNFR2KO) did not affect the half-life of TNF-α mRNA. However, when TTP was also absent (TTP/TNFR1KO, TTP/TNFR2KO), the stability of TNF-α mRNA was increased, particularly when both TTP and TNFR2 were absent. The experiment shown here is representative of 2 independent experiments with cells derived from different groups of animals.

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