Immunofluorescent localization of the Mpl fusion proteins in marrow cells.

Murine marrow cells transduced with either the membrane-targeted Mpl construct (left panel) or the cytoplasmic Mpl construct (right panel) were labeled with an anti-HA monoclonal antibody, biotinylated goat anti–mouse IgG, and streptavidin-FITC and then were analyzed by confocal microscopy. Nuclei were stained with propidium iodide.