IL-12 added to CI-activated DCs does not inhibit Th2 development.

(A) CI-activated DCs were cocultured with 1 ng/mL rhIL-12 for 24 hours, washed, and cocultured with allogeneic CD45RA CD4⁺ T cells for 6 days. T cells were harvested and restimulated with anti-CD3 and anti-CD28 for 24 hours, the supernatants were harvested, and IL-5 and IFN-γ production were measured by ELISA. (B) CI-activated DCs were treated with rhIL-12 for 24 hours, and MIP-1β production was measured by ELISA in the harvested supernatants. Results are representative of 5 different experiments.