Fig. 4.
Immunization scheme of BALB/c mice.
Mice were immunized with 4 μg bsAb BiLu followed by 5 × 104 irradiated A20-EpCAM cells given intraperitoneally 2 hours later. Then, after 4 weeks an identical booster immunization was performed, and finally mice were intraperitoneally challenged another 2 weeks later with 7 × 105 A20 wild-type cells. The role of the T cells was investigated by antibody-induced depletion of CD4+ or CD8+ T cells with 400 to 750 μg CD4.2 antibody and 500 μg CD8.2 antibody, respectively, at indicated time points. From all mice blood samples were collected before any treatment (control sera) as well as 2 days before tumor challenge.
