Induction of cytokines by IL-12 and IL-18 in T and B cells and splenic adherent cells.

Whole spleen cells of BALB/c mice were separated into CD4⁺ and CD8⁺ T cells, and CD43⁻ B cells by MACs. Cells (2 × 10⁶cells) were treated with IL-12 (10 ng/mL), IL-18 (300 ng/mL), or both (IL-12, 10 ng/mL and IL-18, 100 ng/mL) for 72 hours, and indicated cytokines in the medium were measured by specific ELISA. Columns and bars express mean and SE of 5 experiments, respectively. None, not stimulated; ND, not detected.