RT-PCR analysis of messenger RNAs of murine IL-18Rα, IL-18Rβ, and Fc-γ RI in CD4⁺ and CD8⁺ T cells, CD43⁻ B cells, and splenic adherent cells.

CD4⁺ and CD8⁺ T cells and CD43⁻ B cells were isolated by magnetic cell sorter. (A) The purity of the cells was analyzed by flow cytometry after staining with cell-specific surface markers. (B) Messenger RNAs of IL-18Rα and β, Fc-γ RI, and GAPDH were analyzed by RT-PCR using specific primer sets. Fc-γ receptor I mRNA was analyzed to assess the contamination of NK cells and macrophages in T and B cells. Messenger RNA of LNK cell was used as a positive (P) control. For negative control (N), LNK mRNA was subjected to PCR reaction without prior RT reaction.