Fig. 5.
Expression of messenger RNAs of murine G-CSF and IL-18Rα and IL-18Rβ in splenic adherent cells following stimulation with IL-18.
Splenic adherent cells (2 × 107 cells) were treated with IL-18 (100 ng/mL) for 6 hours, and messenger RNAs of G-CSF, IL-18Rα, IL-18Rβ, and GAPDH were analyzed by RT-PCR. Messenger RNA of lipopolysaccharide (LPS)-stimulated peritoneal macrophages was used as a positive (P) control. For negative control (N), the above mRNA was subjected to PCR reaction without prior RT reaction.
