Fig. 4.
Effect of IL-18 on in vitro cytokine formation in wild and IFN-γ deficiency with or without IL-12.
Whole spleen cells isolated from wild and IFN-γ–deficient mice (5 × 106 cells each) were treated for 72 hours with various concentrations of IL-18 (0.1-1000 ng/mL). GM-CSF (A), IL-5 (B), IL-6 (C), and IFN-γ (D) contents in culture supernatant were measured by specific ELISA. Closed and open circles show cytokines secreted by cells incubated with IL-18 with and without IL-12, respectively. Bars represent SE of 4 experiments, using one mouse per experiment.
