Detection of hematopoietic cells in the initial cell preparations from fetal brain tissues and in EGF-generated primary and secondary neurospheres.

Antibodies against CD45 and HLA class I antigens (HLA-A, B, C) were used to analyze the samples for the possible contamination of human hematopoietic cells. Human fetal BM cells from the same donors of brain tissues were used as the positive controls for staining of CD45 and HLA-A, B, C hematopoietic markers. FB indicates the initial fetal brain cell suspensions. 1°NSC indicates EGF-generated primary neurospheres. 2°NSC indicates EGF-generated secondary neurospheres. Auto indicates the autofluorescence control of these samples.