Single primary EGF-generated human neurospheres contain neurons, astrocytes, and oligodendrocytes.

Individual EGF-generated primary human neurospheres were plated on poly-L-ornithine–coated glass coverslips in differentiation cultures for 7 to 8 days in vitro. (A) An example of a single EGF-generated primary human neurosphere growing on a poly-ornitine–coated glass coverslip for one week in differentiation culture. Triple-labeled immunocytochemical staining of the differentiated neurosphere with antibodies to GFAP (B), MAP-2 (C), and O4 (D) revealed cells with the antigenic and morphologic characteristics of astrocytes (B), neurons (C), and oligodendrocytes (D). Scale bars: (A) 25 μm; (B-D) 5 μm.