Expression of IL-7R on DP CD3⁻, immature (intermediate and TN), and SP CD4⁺ thymocytes.

Cells were isolated as described in “Materials and methods” and were maintained in culture in medium alone for 1 day. Immunostaining of IL-7R was analyzed by flow cytometry. Percentage of labeled cells and mean fluorescence intensity (MFI) are indicated in each quadrant. The experiment presented here is representative of independent experiments carried out with thymuses from 3 to 4 different donors. Dotted line indicates isotypic control; solid line, IL-7R.