Fig. 4.
IL-5 inhibits and STS accelerates the externalization of PS and loss of ΔΨm during spontaneous apoptosis of peripheral blood eosinophils.
Eosinophils were cultured in the presence of IL-5 (10−10M, ■), STS (10−5 M, ○), or medium alone (▵) for the indicated times. Apoptosis was assessed by PS exposure and ΔΨm as described in “Materials and methods.” IL-5 inhibited PS externalization and loss of ΔΨm observed in spontaneous eosinophil apoptosis after 24 hours (*P < .05). STS accelerated PS exposure and loss of ΔΨm in eosinophils after 2 hours (‡P < .01) and was almost maximal after 6 hours (*P < .05). Results are expressed as mean ± SE of 3 separate experiments.
