Tat-dominant-negative N 17 Ras construct.

The dominant-negative N17 H-Ras gene was cloned into the pTat-HA vector as described in “Materials and methods.” Six His residues precede the 11-amino acid Tat peptide. Another 10 amino acids of the HA tag and 6 Gly are immediately N-terminal to the N17 Ras protein. Tat-GFP also contains the HA tag (see “Materials and methods”).