GM-CSF–mediated up-regulation of G-CSF receptor RNA in the fetal liver cell lines.

Total RNA extracted from the indicated cell types were subjected to Northern blot hybridization (8 μg/lane) with the indicated probes. Lanes 1 to 5 harbor RNAs extracted from (1) FL-1(+/−) cultured in SCF and IL-3, (2) FL-1(+/−) cultured for 4 days in GM-CSF alone, (3) FL-8(−/−) cultured in SCF and IL-3, (4) FL-8(−/−) cultured in GM-CSF alone for 4 days, and (5) 32D cells cultured in IL-3 alone.