Morphology of the cultured fetal liver cell lines derived from C/EBPα(+/−) and C/EBPα(−/−) mice.

Cytospin preparations of the FL-1(+/−) and FL-8(−/−) cell lines cultured in the indicated growth factors were stained with Wright-Giemsa. Parental SCF + IL-3 cultured FL-1 and FL-8 (A,B). For GM-CSF stimulation (C, D), cells growing in SCF and IL-3 were washed and resuspended in GM-CSF alone and were cultured for 4 additional days. ATRA stimulation (10 μM) was for 5 days (E, F).