The adhesion of macrophages modulate their proliferation.

(A) Macrophages adhere preferably to fibronectin- or vitronectin-precoated surfaces. Cells (10 000) were cultured for 60 minutes on plates precoated with 10 μg/mL BSA (control), 10 μg/mL of the indicated components of the ECM, or 25 μg/mL P-OH-M, an inhibitor of cell adhesion. Macrophage adhesion was analyzed by crystal violet staining. Each determination was made in triplicate, and the values represented correspond to the mean ± SD of 3 independent experiments. (B) Adhesion modulates macrophage proliferation. Macrophages adhered to plates precoated with 10 μg/mL BSA, laminin or fibronectin, or 25 μg/mL P-OH-M were stimulated with the indicated concentrations of M-CSF and their proliferation was analyzed by [³H]-thymidine incorporation after 24 hours. Each point was made in triplicate, and the values represented correspond to the mean ± SD of 2 independent experiments.