Morphologic and phenotypical characterization of the C2174PBL cells.
(A,B) C2174PBL cell line cytospins after 20 months of culture; note the presence of multinucleated giant cells (Giemsa staining). Original magnification of each, × 40. (C) An aliquot of the C2174PBL cultured PBMCs permeabilized and then stained with rabbit specific antibodies (CD3 FITC, CD8 FITC) and EBV-LMP1 antibody (LMP1 PE) was fixed in paraformaldehyde placed in mounting media and analyzed using a Leica DMRA microscope (using program QFISH). Original magnification, × 63. The upper portion of panel C shows a double-positive cell for LMP1 and CD3. The dent present on the upper right corner clearly shows evidence of an adjacent double-negative cell. The middle panel shows an LMP1-positive and a CD8−cell, whereas the lower panel shows a CD3+ and an LMP1-negative cell. (D) Histogram plots from the FACS analysis of the C2174 cultured PBMCs at 20 months of culture. Blue lines indicate specific isotypic control.
