Relative survivin expression in UCB CD34⁺cells and leukemic cells.

(A) Multivariate intracellular flow cytometry plots of survivin expression in fresh UCB CD34⁺ cells, and exponentially growing HL-60 and MO7e leukemia cells. Survivin MCF is shown in each data plot. Data are from 1 of 2 experiments with identical results. (B) Western blot analysis of survivin levels in freshly isolated UCB CD34⁺ cells; CD34⁺ cells stimulated with 100 ng/mL each of Tpo, FL, and SCF for 48 hours; and exponentially growing HL-60 and MO7e leukemia cells. Cell lysates from 1 × 10⁶cells were loaded in each lane and blotted with polyclonal anti–human survivin. Data are from 1 of 3 experiments.