VEGF antisense specifically inhibits VEGF.

(A) Effect of AS-3 and mutant AS-ODNs on the viability of KS Y-1 cells in vitro. Cells were seeded at 1 × 10⁴ cells per well in 24-well plates and treated with the ODNs as indicated on days 1 and 3. Cell viability was performed on day 5 by MTT assay. Results represent the mean ± SE of quadruplicate samples. We also tested a previously described VEGF AS ODN, M3.64 (B) Effect of AS-3 and mutant AS-ODNs on the production of VEGF and IL-8. Cells were cultured in media supplemented with 2% FCS for these experiments. Cells were treated with various concentrations of the oligonucleotides at hours 0 and 16. The supernatants were collected at hour 24 and assayed for VEGF and IL-8 using ELISA kits. Results are presented as median of replicate experiments ± SE.