Fig. 2.
![Fig. 2. Reactivity of PA autoantibodies with KO variant GPIIb-IIIa. / (A) Reaction of mAbs (AP3 and OP-G2) and antiplatelet alloantibodies (anti–HPA-1a and anti–HPA-3a) with KO variant GPIIb-IIIa. (B) PA autoantibodies from 11 patients with chronic ITP, showing a marked decrease (greater than 50%) in the reactivity against KO variant compared with WT GPIIb-IIIa. Results are expressed as ΔOD: [OD value obtained from a test sample − (mean + 3 SD) OD value obtained from 5 control samples]. Open columns represent ΔOD value in antigen-capture ELISA using WT GPIIb-IIIa, and filled columns represent ΔOD value in antigen-capture ELISA using KO mutant. Data are representative of 2 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/6/10.1182_blood.v98.6.1819/7/h81811514002.jpeg?Expires=1723422296&Signature=45-QhEFL5GSsHojMod5Y9cJWtwnlnDGPu6FQioMKhBC4CZ4ut15-2wmxaxKTDhnRXacETDI4Q33x0Z~sJHDZAoMa0Ks75RGkaXpo7JzkBdsvGYeR8W0juSj7EYLNMiyBMC6lpRmTeVnD2RhSDsOVqw0pIXJ5adT-quzwBBqtbJ4eh9noI472SUCfeL4ad5KA2j26XzdXbzkodMiEY6iwaz3qUS7goaEctQYthhPzHI1MA2Uh-AqRFwryErXPP4MoDdspuwl7L61kAjgTaOhwObd-TAjzJvQ3Jtps7OZsV2GCwoqsiVDiKeKRHhFzumnt8aFLAxarUQT3Ukv4c5bSTw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Reactivity of PA autoantibodies with KO variant GPIIb-IIIa.
(A) Reaction of mAbs (AP3 and OP-G2) and antiplatelet alloantibodies (anti–HPA-1a and anti–HPA-3a) with KO variant GPIIb-IIIa. (B) PA autoantibodies from 11 patients with chronic ITP, showing a marked decrease (greater than 50%) in the reactivity against KO variant compared with WT GPIIb-IIIa. Results are expressed as ΔOD: [OD value obtained from a test sample − (mean + 3 SD) OD value obtained from 5 control samples]. Open columns represent ΔOD value in antigen-capture ELISA using WT GPIIb-IIIa, and filled columns represent ΔOD value in antigen-capture ELISA using KO mutant. Data are representative of 2 separate experiments.
