Tissue distribution of murine TfR2.

(A) RT-PCR analyses. RT-PCR was performed with primers for murine TfR2 (32 cycles) as well as GAPDH (24 cycles). Mouse multiple cDNA panels were used as templates. Whole-mouse cDNA and dH₂O were used as positive and negative controls, respectively (right 2 lanes). The products were subjected to electrophoresis, Southern blotting, hybridization with radiolabeled probes, and autoradiography. D indicates day. (B) Northern blot analysis of various murine tissues. Various murine tissues were prepared from C3H mice, and total RNA (5 μg/lane) was extracted and Northern blotted. (C) Expression profiles of TfR1 and TfR2 in the liver and spleen during development. Spleens and livers were excised from day 13 and day 18 embryos, from postnatal day 1 and day 4 mice, and adult mice. (B-C) Total RNA was extracted, and expression levels of TfR1, TfR2, and GAPDH were compared by Northern analysis. (D) Relative expression levels of TfRs in the liver and spleen during development are calculated by an image analyzer, standardized by the values of GAPDH, and shown as bar graphs (■, TfR1; , TfR2). The values of postnatal day 1 for spleen and day 13 embryo for liver are adjusted to 1.0.