Surface expression of GPIb on recombinant cell lines.

Transfected cell lines were incubated with the anti-GPIbα mAbs (A) AK3 and (B) AK2. Cell autofluorescence was determined by use of a mouse isotype control antibody. Bound mAbs were detected using an FITC-labeled anti–mouse IgG antibody, followed by flow cytometric analysis. Mean fluorescence data are plotted ± SEM.