Fig. 5.
Fig. 5. NFAT1 is required for optimal IFN-γ production by T cells at all stages of T-cell differentiation. / NFAT1 is required for optimal IFN-γ production by naive and differentiated T-helper cells. NFAT1+/+IL-4−/− and NFAT1−/− IL-4−/−CD4+ T cells were sorted by FACS for high expression of Mel-14 and were cultured with plate-bound anti-CD3 and IL-2 for 2 consecutive rounds of differentiation, in the absence (top panels) or presence (bottom panels) of IL-12. Before differentiation (stimulation 1, left panel) and after the first (stimulation 2, middle panels) and second (stimulation 3, right panels) rounds of differentiation, cells were stimulated for 48 hours (stimulation 1) or 24 hours (stimulations 2 and 3) with plate-bound anti-CD3, after which IFN-γ was analyzed in the supernatants of the cells. Note that y-axis scales representing IFN-γ production are different in all panels.

NFAT1 is required for optimal IFN-γ production by T cells at all stages of T-cell differentiation.

NFAT1 is required for optimal IFN-γ production by naive and differentiated T-helper cells. NFAT1+/+IL-4−/− and NFAT1−/− IL-4−/−CD4+ T cells were sorted by FACS for high expression of Mel-14 and were cultured with plate-bound anti-CD3 and IL-2 for 2 consecutive rounds of differentiation, in the absence (top panels) or presence (bottom panels) of IL-12. Before differentiation (stimulation 1, left panel) and after the first (stimulation 2, middle panels) and second (stimulation 3, right panels) rounds of differentiation, cells were stimulated for 48 hours (stimulation 1) or 24 hours (stimulations 2 and 3) with plate-bound anti-CD3, after which IFN-γ was analyzed in the supernatants of the cells. Note that y-axis scales representing IFN-γ production are different in all panels.

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