Structure-activity relationships for the effects of PGD₂ and related compounds on CD11b expression and eosinophil migration.

(A) Human leukocytes were incubated with PGD₂ (●; n = 12), BW245C (▪; n = 10), PGJ₂ (▵; n = 6), and 13,14-dihydro-15-oxo-PGD₂ (○; n = 7), followed by measurement of CD11b expression in eosinophils as illustrated in Figure2. The values are means ± SE and are expressed as percentages of the maximal responses to PGD₂ after subtraction of the values obtained from unstimulated cells. (B) The chemotactic effects of PGD₂ (●), BW245C (▪), and PGJ₂ (▵) on purified human eosinophils were evaluated as described in the legend to Figure 1. The results are means ± SE of determinations on cells from 6 individuals and are expressed as percentages of the maximal responses to PGD₂.